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. 2012 Sep 25;7(9):e46233. doi: 10.1371/journal.pone.0046233

Figure 3. PorA loop 4 and OmpP2 loop 2 peptides bind LamR.

Figure 3

(A) Binding of LamR to OmpP2 loop 2 (labeled L2), OmpP2 loop 2scr (L2scr), PorA loop 4 (L4), PorA loop 4scr (L4scr) or PorA loop 1 (L1) coated ELISA plates. Results shown are means of triplicate wells from a representative example from two independent experiments. (B) Binding of DIG-labeled LamR to PorA20–392 in the presence of 0.5, 10, 50 or 100 µg of peptide corresponding to PorA loop 4 (L4) or loop 1 (L1). The binding of LamR to PorA20–392 in the absence of peptide served as a negative control for inhibition. * p<0.05 compared to binding in the absence of peptide. ** p<0.01 compared to binding in the absence of peptide. Data shown are means from three independent experiments; in each experiment each sample was tested in triplicate. Error bars indicate SEM.