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. 2012 Sep 25;7(9):e46236. doi: 10.1371/journal.pone.0046236

Figure 4. The CD95 self-association domain covers the residues 43 to 66. A.

Figure 4

Schematic representation of the different CD95-mCherry constructs. B. Supernatants from HEK cells transfected with the constructs depicted in A were fractionated by gel filtration using a S-300 HR column. Seventeen fractions were harvested and analyzed by immunoblot analysis (anti-DsRed). C. Densitometry analyses were performed on the immunoblots shown in B using Fiji software. For each construct, the estimated quaternary structure is depicted. D. Supernatants from HEK cells transfected with the constructs depicted in A were fractionated by gel filtration using a S-200 HR column. Twenty-one fractions were harvested and analyzed by immunoblot analysis (anti-DsRed). E. Densitometry analyses were performed on the immunoblots shown in D using Fiji software. For each construct, the estimated quaternary structure is depicted.