Table 1. COMMD7 shRNA sequences and Primer pairs for polymerase chain reaction.
Gene | Primer Sequence (5′ to 3′) | Size of PCR Product (bp) | |
Sense | Anti-sense | ||
COMMD7 shRNA | GATCCGCTCTGGGTCTTAGTGAGGATTCAAGACGTCCTCACTAAGACCCAGAGTTTTTTGTCGACA | GCGAGACCCAGAATCACTCCTAAGTTCTGCAGGAGTGATTCTGGGTCTCAAAAAACAGCTGTTCGA | |
COMMD7 | AGTGGCTTTCTCCTCACTAAGACC | GGAAAGATTTCTGGCTCAGCTC | 181 bp |
β-actin | CCAACCGCGAGAAGATGA | ACCAGAGGCGTACAGGGAT AG | 98 bp |