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. 2012 Sep 25;7(9):e46185. doi: 10.1371/journal.pone.0046185

Figure 1. Inducible expression system of wild-type and mutant SOD1s in NSC-34 cells.

Figure 1

A: NSC-34 cells were stably transduced with an inducible lentivirus expressing human Myc-tagged wild-type or mutant SOD1 protein. Cells were cultured with or without doxycycline (Dox, 2 µg/ml) for 48 h to induce SOD1 protein. Tubulin is shown as a loading control. hSOD1 and mSOD1 indicate human SOD1 and mouse endogenous SOD1, respectively. B: Cell viability assay based on the MTS method showed that overexpression of both types of mutant SOD1, G93A and G85R, caused cytotoxicity in serum-free culture medium. Mock indicates mock-transfected NSC-34 cells. Data are presented as mean ± SEM. Statistics were evaluated using 1-way ANOVA with Dunnett's post-hoc test. *P<0.05, **P<0.01 C: Cytotoxicity detection assay using the LDH release method revealed that overexpression of both types of mutant SOD1, G93A and G85R, caused cytotoxicity in serum-free culture medium. Data are presented as mean ± SEM. Statistics were evaluated using 1-way ANOVA with Dunnett's post-hoc test. *P<0.05, **P<0.01.