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. 2012 Sep 25;7(9):e44619. doi: 10.1371/journal.pone.0044619

Figure 3. Vaccination/challenge experiment with serotyped BTV viruses.

Figure 3

On day 0 (0 dpi), four sheep were intramuscularly (i.m.) vaccinated in the neck with 1 ml of 105 TCID50/ml BTVac-1, 1 ml of 101.4 TCID50/ml BTVac-6, or 1 ml of 105 TCID50/ml BTVac-8. A fourth group was vaccinated i.m. with 1 ml in total consisting of 0.33.105 TCID50/ml BTVac-1, 0.33.101.4 TCID50/ml BTVac-6 and BTVac-8. On 21 dpv (arrow), all sheep, including the control group were challenged subcutaneously (s.c.) with 1 ml of 105 TCID50/ml of BTV8/net07/e1/bhkp3. (A) body temperatures (mean values per group; lines) were recorded daily. (B) BTV was determined by the panBTV-PCR test (mean values per group). (C) BTV-VP7 directed antibodies in serum samples were detected with the panBTV blocking ELISA. The mean blocking percentage per group was displayed as 100-value (sample). (D) Clinical scores were observed daily and displayed as total scores per group (bars per group).