Table 1. Primers used for differential detection of genome segment Seg-2 (S2-genotyping) and Seg-6 of BTV serotype 1, 6, and 8.
| namea | sequence | expected amplicon size (bp) |
| BTV1VP2/283-1F | TTGTTGAAAGTACGAGACACAAGAG | 175 |
| BTV1VP2/457-1R | GTATCAGCCTTCTTTGAATCGATT | |
| BTV1.1VP2 probe | CATCCACTGCACCCACTGGTCA | |
| BTV6VP2/1757-5F | AGGAACAGTCGGCTTATCAC | 192 |
| BTV6VP2/1948-5R | TTCGCTAATGTGCTTCTCCAT | |
| BTV6.5 VP2 probe | TTGTCAGCTTTACGCAAACCCCG | |
| BTV8VP2/1873-4F | CGGAGACAGCGCAGTATGTA | 225 |
| BTV8VP2/2097-4R | CCTCGGTAGTATCCCTCACG | |
| BTV8.4 VP2 probe | ACATACGATGCCYTCGGAGGATTCTG | |
| BTV1S6F1 | AGTGATCAATGCTTTAAGCGGG | 831 |
| BTV1S6R | GTAAGTGTAAGTGCTTCCCGTC | |
| BTV6S6F1 | GTTAAAAAGATCCCCATGAT | 490 |
| BTV6S6R4 | CGCTTTACAGAGCACCTTAT | |
| BTV8S6F | GTTAAAAAAGCGATCGCTTTCG | 638 |
| BTV8S6R4 | GCCTCTTTTAACGCATCG |
a. Primers were purchased with Eurogentec Benelux. Probes were purchased with TibMolBiol Berlin and are labeled with FAM at the 5′-end, and with the black hole quencher (BHQ) at the 3′-end.