Table 2. Genotyping results from vaccinated and challenged animals with serotype specific PCR tests on Seg-2 (S2-genotyping) and restriction enzyme analysis of amplicons of the panBTV-PCR test (S10-genotyping).

Group (n = 4)		S2-genotyping (number of positive animals)a									S10–genotypingb
		11 dpv			21 dpv			14 dpc			7 dpc		11 dpc		14 dpc
	Serotype	1	6	8	1	6	8	1	6	8	BT Vac-x	BTV8	BT Vac-x	BTV8	BT Vac-x	BTV8
BTVac-1	1	4	0	0	4	0	0	4	0	0	+		+		+
BTVac-6	6	0	4	0	0	4	0	0	4	0	+		+		+
BTVac-8	8	0	0	4	0	0	3	0	0	3	+		+		+
CombiVac	1, 6, 8	4	0	4	3	1	3	2	2	3	+		+		+
Control	8	0	0	0	0	0	0	0	0	4		+		+		+

a. S2-genotyping was based on a serotype specific PCR-test with specific primer sets listed in table 1 [14].

b. S10-genotyping was based on the panBTV-PCR test used for diagnostic purposes [23]. The amplicon was digested with specific restriction enzymes and analyzed on an agarose gel, see materials and methods.