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. 2012 Sep 25;7(9):e44806. doi: 10.1371/journal.pone.0044806

Figure 1. uPAR carrying S90E and S90P substitutions. A.

Figure 1

: Arg91 and Tyr92 superposition of the backbone atoms of the various 88–92 segments of SuPAR from x-ray structures. Residue 89 is not visible in the electron density map of 1YWH. Side chains are reported as stick, Arg residues are in blue. Backbones are reported as ribbon drawing: Yellow, 2I9B, chain E and F, residues 88–92; Red, 3BT2, chain U, residues 88–92; Mauve, 1YWH, chain E, residues 89–92; Cyan, 1YWH chain M, residues 89–92; Purple, 1YWH chain A, residues 89–92. B. Lysates (25 µg/sample) from 293 stably transfected either with pcDNA3 empty vector (293/mock) or pcDNA3 coding for human uPAR (293/uPARwt, uPARS90E, uPARS90P), were resolved on a 10% SDS-PAGE followed by Western blotting with R4 anti-uPAR or anti-αv mAbs. C. Cytofluorimetric analysis of the indicated stably transfected 293 clones with R4 anti-uPAR mAb or nonimmune immunoglobulins. D. Representative images of the indicated stably transfected 293 clones immuno-stained with anti-uPAR R4 mAb.