Figure 13. Silencing of Orai1, Stim1, and TRPC1 reduces store-operated Ca²⁺ entry in endothelial progenitor cells.

A, SOCE evoked by CPA (10 µM) in RCC-EPCs transfected with a control siRNA (black tracing) or with a specific siRNA sequence devised to knock down either Orai1 (grey tracing) or Stim1 (light gray tracing). B, mean±SE of the amplitude of CPA-induced Ca²⁺ release and CPA-induced SOCE under each condition described in A. The asterisk indicates p<0.05. C, SOCE triggered by CPA (10 µM) in N-EPCs transfected with a scrambled shRNA (black tracing) and with a shRNA selectively targeting TRPC1 (grey tracing). D, mean±SE of the amplitude of CPA-induced Ca²⁺ release and CPA-induced SOCE under each condition described in C. E, SOCE triggered by CPA (10 µM) in RCC-EPCs transfected with a scrambled shRNA (black tracing) and with a shRNA selectively targeting TRPC1 (grey tracing). F, mean±SE of the amplitude of CPA-induced Ca²⁺ release and CPA-induced SOCE under each condition described in E.