Table 2. Primer sequences used for real time reverse transcription/polymerase chain reaction of Orai1-3, Stim1-2, TRPC1-7.

Gene	Primer sequences		Size (bp)	Accession number
Orai1	Forward	5′-AGTTACTCCGAGGTGATGAG-3′	257	NM_032790.3
	Reverse	5′-ATGCAGGTGCTGATCATGAG-3′
Orai2	Forward	5′-CCATAAGGGCATGGATTACC-3′	334	NM_001126340.1variant 1
	Reverse	5′-CAGGTTGTGGATGTTGCTCA-3′		NM_032831.2variant 2
Orai3	Forward	5′-CCAAGCTCAAAGCTTCCAGCC-3′	159	NM_152288.2
	Reverse	5′-CAAAGAGGTGCACAGCCACCA-3′
Stim1	Forward	5′-CCTCAGTATGAGGAGACCTT-3′	347	NM_003156.3
	Reverse	5′-TCCTGAAGGTCATGCAGACT-3′
Stim2	Forward	5′-AAACACAGCCATCTGCACAG-3′	186	NM_020860.2
	Reverse	5′-GGGAAGTGTCGTTCCTTTGA-3′
TRPC1	Forward	5′-ATCCTACACTGGTGGCAGAA-3′	307	NM_003304.4
	Reverse	5′-AACAAAGCAAAGCAGGTGCC-3′
TRPC3	Forward	5′-GGAGATCTGGAATCAGCAGA-3′	336	NM_001130698.1 variant 1
	Reverse	5′-AAGCAGACCCAGGAAGATGA-3′		NM_003305.2 variant 2
TRPC4	Forward	5′-ACCTGGGACCTCTGCAAATA-3′	300	NM_016179.2 variant alpha
	Reverse	5′-ACATGGTGGCACCAACAAAC-3′		NM_001135955.1 variant beta
				NM_001135956.1 variant gamma
				NM_001135957.1 variant delta
				NM_003306.1 variant epsilon
				NM_001135958.1 variant zeta
TRPC5	Forward	5′-GAGATGACCACAGTGAAGAG-3′	221	NM_012471.2
	Reverse	5′-AGACAGCATGGGAAACAGGA-3′
TRPC6	Forward	5′-AGCTGTTCCAGGGCCATAAA-3′	341	NM_004621.5
	Reverse	5′-AAGGAGTTCATAGCGGAGAC-3′
TRPC7	Forward	5′-CACTTGTGGAACCTGCTAGA-3′	387	NM_020389.1
	Reverse	5′-CATCCCAATCATGAAGGCCA-3′
β-actin	Hs_ACTB_1_SG, QuantiTect Primer Assay QT00095431, Qiagen		146	NM_001101