Figure 5. Nrg1 determines ALT1 and ALT2 repression.

Northern blot of total RNA obtained from wild type and nrg1Δ strains grown on either glucose-ammonium (A) or glucose-alanine (B). Samples were collected from various OD₆₀₀ as stated. Representative results from three experiments are shown. ChIP assays were performed using anti-Myc antibody on wild type strains containing myc¹³ epitope-tagged NRG1. As a control, equivalent samples were treated with anti-HA antibody. Strains were grown on 2% (w/v) glucose +7 mM alanine to either 0.3 or 1.8 OD₆₀₀, cells were centrifuged, collected and used to prepare samples for ChIP (C, D). PCR was performed with the deoxyoligonucleotides described in Table 3. As a negative control, PCR was also performed for LEU4 coding region. Results are representative of three independent experiments.