Table 1. Characteristics of ligands, coatings, and fluorophores.
| Mw (Da) | pI | GRAVY1 | Aliphatic index2 | Net charge at pH 7 | |
| Ligand | |||||
| EGF | 6,045 | 4.69 | −0.506 | 55.09 | −2.2 |
| HEWL | 14,313 | 9.32 | −0.472 | 65.12 | 7.7 |
| HER2 Affibody | 6,736 | 8.9 | −0.625 | 84.58 | 2 |
| HER1 Affibody | 13,860 | 4.65 | −0.507 | 79.68 | −5 |
| Coating | |||||
| Poly-L-Lysine | variable (150,000–300,000) | 9.6 | −3.9 | 0 | poly-cation |
| Fibronectin | 269,110 | 5.28 | −0.513 | 69.06 | −66 |
| Laminin3 | ∼700,000 | ∼5.3 | ∼−0.5 | ∼70 | −227 |
| Collagen4 | ∼300,000 | ∼7.8 | −0.261 to 0.919 | ∼40 to 80 | 35 |
| BSA | 66,433 | 5.6 | −0.475 | 76.14 | −17 |
| FCS | mixture of proteins | variable | variable | variable | variable |
| Linear PEG | 5,000 | – | – | – | 0 |
| Star PEG | 10,000 | – | – | – | 0 |
| Nanogel | mixture of Star PEG and BSA | – | – | – | – |
| Fluorophore | |||||
| Alexa Fluor 488 | 643 | – | – | – | −2 |
| Alexa Fluor 546 | 1,079 | – | – | – | −2 |
| Atto 647N | 868 | – | – | – | 1 |
1
GRAVY is the grand average of hydropathicity; more negative values indicate greater hydrophilicity [58].
2
Aliphatic index; the relative volume occupied by aliphatic side chains [59].
3
Laminin values taken as average of α1, β1, and γ1 chains.
4
Collagen values representative of the range of collagen types as commercially prepared dishes do not specify type. – indicates not applicable or not known.