Progressive and reversible walking demonstrated by FRET assay. Dabcyl (quencher, star)-labeled walker moves on the anchorage site labeled with fluorophore TAMRA (circle) and FAM (triangle). Fluorescence intensity (515nm for FAM, λex= 488nm; 580nm for TAMRA, λex= 550nm) was monitored during (a) S1→S2 locomotion (continuous visible irradiation), (b) S2→S1 locomotion (visible irradiation for 1000s followed by UV irradiation), and (c) the reversible movement of the walker during the alternate period of UV and visible irradiation.