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. Author manuscript; available in PMC: 2013 Sep 25.

Published in final edited form as: ACS Nano. 2012 Aug 15;6(9):8007–8014. doi: 10.1021/nn302615f

PEG-HCC treatment rapidly improves CBF in the injured cortex in rats with TBI plus hypotension after a single dose and two sequential doses. Laser Doppler was used to measure CBF given as a percent of the baseline (pre-injury) CBF. Relative CBF below 100 (dashed line) indicate low CBF. Relative CBF after a single drug dose (arrow) administered during the “hospital phase” in the (A) injured cortex (B) peri-lesional cortex, and (C) contralateral cortex; the effect of sequential dosing in a second set of rats treated with drug during the “hospital phase” and again 2 h later (arrows) are shown in (D) injured cortex (E) peri-lesional cortex, and (F) contralateral cortex. Drugs (PEG-HCC or PBS vehicle) were given where indicated. Time 0 min indicates when the TBI was performed. Phase 1 = TBI+hypotension; Phase 2 = saline during “ambulatory phase”; Phase 3 = vehicle or PEG-HCC treatment and blood reinfusion during “hospital phase”. Error bars are S.E.M. Repeated measures ANOVA with Bonferroni post test was used to calculate statistics. * p-value <0.05 and *** p-value <0.01 for vehicle (red) compared to PEG-HCCs (blue). Note that 100% of CBF is considered “normal”. Approximately 50% and 150% are considered low and high cerebral blood flow, respectively.

PEG-HCC treatment rapidly improves CBF in the injured cortex in rats with TBI plus hypotension after a single dose and two sequential doses. Laser Doppler was used to measure CBF given as a percent of the baseline (pre-injury) CBF. Relative CBF below 100 (dashed line) indicate low CBF. Relative CBF after a single drug dose (arrow) administered during the “hospital phase” in the (A) injured cortex (B) peri-lesional cortex, and (C) contralateral cortex; the effect of sequential dosing in a second set of rats treated with drug during the “hospital phase” and again 2 h later (arrows) are shown in (D) injured cortex (E) peri-lesional cortex, and (F) contralateral cortex. Drugs (PEG-HCC or PBS vehicle) were given where indicated. Time 0 min indicates when the TBI was performed. Phase 1 = TBI+hypotension; Phase 2 = saline during “ambulatory phase”; Phase 3 = vehicle or PEG-HCC treatment and blood reinfusion during “hospital phase”. Error bars are S.E.M. Repeated measures ANOVA with Bonferroni post test was used to calculate statistics. * p-value <0.05 and *** p-value <0.01 for vehicle (red) compared to PEG-HCCs (blue). Note that 100% of CBF is considered “normal”. Approximately 50% and 150% are considered low and high cerebral blood flow, respectively.