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MKP-1 is required in BMSC for 1,25-(OH)2D3 (D3) induction of RANKL. A, RT-qPCR analysis of RANKL mRNA expression in response to 18 h treatment with inflammatory stimuli and 10−8 m 1,25-(OH)2D3. B, ELISA of soluble RANKL expression from cultured media containing indicated cytokines, LPS, or 10−8 m 1,25-(OH)2D3 for 48 h (n = 4; ***, P < 0.001). Aa, Aggregatibacter actinomycetemcomitans; Pg, Porphyromonas gingivalis.
