Fig. 4.

EMSA analysis shows 1,25-(OH)₂D₃-stimulated MKP-1^−/− BMSC have a decreased VDRE binding capacity. A, The α-³²P-labeled mOP VDRE was incubated with 1,25-(OH)₂D₃-treated nuclear extracts from WT and MKP-1^−/− BMSC and electrophoresed through a nondenaturing gel. R, RXRα supershifts; V, VDR antibody supershifts; *, the putative mOP-RXRα/VDR heterodimer complex. Results are representative of three independent experiments. B, ChIP analysis of the RANKL VDRE enhancer region located −76 kb from the RANKL TSS (mRLD5) and control non-VDRE region located −50 kb from the RANKL TSS (IS4). Results are representative of three independent experiments. Ctrl, Control.