Fig. 6.
MKP-1 is required for 1,25-(OH)2D3-induced nuclear translocation of the VDR-RXRα heterodimer. WT and MKP-1−/− BMSC were treated with vehicle control or 10−7 m 1,25-(OH)2D3 for 1 h and were subjected to PLA with antibodies against VDR (mouse) and RXRα (rabbit); confocal images were captured for quantification. A, Representative confocal images of PLA signals in WT and MKP-1−/− BMSC. B, Quantification of total and nuclear PLA signals in the Z-range of the DAPI stained nuclei for the indicated treatments. White scale bar, 10 μm (n = 3; *, P < 0.05). Ctrl, Control.