Fig. 7.

MKP-1 is required for maximal 1,25-(OH)₂D₃-induced osteoclastogenesis. WT and MKP-1^−/− BMSC were cocultured with RAW264.7 cells to test the osteoclast-inducing potential of 1,25-(OH)₂D₃-stimulated stromal cells. A, Quantification of osteoclasts per well and nuclei per osteoclast. Coculture osteoclast-resorbing activity was quantified on osteologic discs. B, Representative pit images and C) pits per well and total pit area per well. (n = 3; P < 0.05; **, P < 0.01; ***, P < 0.001). Ctrl, Control; OPG, osteoprotegerin.