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. 2012 Aug 27;109(38):E2523–E2532. doi: 10.1073/pnas.1208141109

Fig. 7.

Fig. 7.

Cofilin S3E affects mitochondrial dynamics and counteracts SRF-VP16. In primary hippocampal neurons (A–H), SRF-VP16 enhanced mitochondrial size and number in neurites (B and F). (C and G) Cofilin S3E reduced mitochondrial size and neurite occupancy. (D and H) Coexpression of cofilin S3E and SRF-VP16 prevented SRF-VP16’ s potential to enhance mitochondrial size and number in neurites. In Srf mutant neurons expressing SRF-ΔMADS-VP16 (K and O), P-cofilin levels were up-regulated compared with WT (I and M). SRF-VP16 reduced P-cofilin levels in WT (J and N) and more pronouncedly in Srf mutant (L and P) neurons. Cofilin S3E reduced mitochondrial (mito.) size (Q) and neurite occupancy (R) and counteracted SRF-VP16’s impact on mitochondria. Quantification of phosphocofilin (S) and total cofilin (T) levels in individual neurons is shown. (*P < 0.05; **P < 0.01; ***P < 0.001.) Numbers in bars represent numbers of independent experiments. ko, knockout; n.s., not significant; wt, wild type. (Scale bar: A–P, 10 μm.)