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Electrophoretic gel (Agarose) analysis of the amplicon from (A) the real-time PCR on SAW and (B) the conventional thermocycler. (L) DNA ladder 100 bp, (1, 2 and 5) noninfected mouse blood, (3, 4 and 6) infected mouse blood lysed with SAW, (7) infected mouse chemically isolated DNA, (8) no template. The gels show specific amplification of the predicted target DNA fragments at 64 bp, in agreement with conventional PCR. (C) The Ct value as a function of parasite concentration.
