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UTX controls mesodermal differentiation of ES cells independent of H3K27 demethylase activity. (A) Microscopic pictures of flox, KI, and KO ES cells before differentiation and EBs at day 4, day 8, and day 12 of differentiation. (B) qRT-PCR analysis of ES cell markers (Nanog, Oct4), mesoderm markers (Brachyury, Wnt3) and endoderm markers (Gata4, Gata6) at indicated time points of differentiation. D2, D4, D8, and D12 refer to day 2, 4, 8, and 12, respectively.
