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. 2012 Sep 4;109(38):15307–15311. doi: 10.1073/pnas.1201632109

Fig. 2.

Fig. 2.

β-Cleavage of APP is not specifically affected by genes involved in LOAD susceptibility. (A) HeLa swAPP cells were transfected with siRNAs to the corresponding genes, and the supernatant was analyzed for sAPPβ 72 h after transfection (with 3 h medium exchange) using duplex ECL array (MSD). The values are given as ECL counts normalized to cell viability and relative to that of a scrambled (MedGC) control. The genes (BIN1, CLU, CR1, PICALM, CD33, and CD2AP) are among the top 10 AlzGene meta-analysis results (ranking is based on P value, and top 10 have P values <0.00001) listed in the AlzGene database. APP, BACE1, Pen-2, and scrambled (MedGC) serve as both transfection and assay controls. (B) Cell viability: HeLa swAPP cells were incubated for 72 h after siRNA transfection and subjected to a cell viability assay using AlamarBlue. Results are shown as fluorescence counts using a microplate absorbance/fluorometer plate reader (Molecular Devices Spectramax Gemini XS). Effects of positive (KIF11) and negative (scrambled) controls are shown among the other corresponding silenced genes. Note that only Kif11 silencing produces dramatic effects in cell viability.