Fig. 4.

GDP-l-fucose is not supplied through body fluids. (A–C) AAL staining and (A′–C′) bright-field images of late third-instar eye imaginal discs (indicated by white square brackets). (A and A′) Wild-type eye imaginal disc. (B and B′) Gmd^H78 homozygous eye imaginal disc. (C and C′) Eye imaginal disc of a Gmd^H78 homozygote overexpressing UAS-Gmd driven by GMR-Gal4. (D and E) Anti-Wg antibody staining of late third-instar wing imaginal discs. AD, antenna imaginal discs. (D) Wing imaginal disc isolated from the larva of a Gmd^H78 homozygote overexpressing UAS-Gmd in the eye imaginal disc driven by GMR-Gal4. (E) Wing imaginal disc isolated from the larva of a Gmd^H78 homozygote overexpressing UAS-Gmd in the fat body driven by Lsp-Gal4. White arrowheads indicate the D/V boundary. (Scale bar in A, 50 μm, applicable to A–C′; in D, 50 μm, applicable to D and E.)