Fig. 5.

inx2 is required for the intercellular delivery of GDP-l-fucose. (A–O) Late third-instar wing imaginal discs stained with an anti-GFP antibody (A, E, and L, green), AAL (B, F, J, and M, magenta), and an anti-Cut antibody (C, G, K, and N, turquois). (A–D) ptc-Gal drove the expression of two UAS-GFP transgenes where a hairpin dsRNA of Gmd (Gmd IR) was produced. (E–H) ptc-Gal drove the expression of two UAS-GFP transgenes where a hairpin dsRNA of inx2 (inx2 IR) was produced. (I–L) ptc-Gal drove the expression of a UAS-GFP transgene where hairpin dsRNAs of Gmd and inx2 were produced. (M–O) ptc-Gal drove the expression of UAS-inx2 where hairpin dsRNAs of Gmd and inx2 were produced. The total number of UAS promoters in each experiment was adjusted to be the same by introducing UAS-GFP. D, H, L, and O are merged images of B and C, F and G, J and K, and M and N, respectively. White arrowheads indicate the regions of reduced AAL staining (J and K) and cut expression (K and L). (P–S) Adult wings. (P) Wild-type. (Q–S) Wings producing hairpin dsRNAs targeting Gmd (Q), inx2 (R), or Gmd and inx2 (S) under the control of ptc-Gal4. Square bracket and black arrow indicate a wing blade notch and wing vein-thickening, respectively, in S. (Scale bar in A, 50 μm, applicable to A–O.)