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. 2012 Aug 27;109(38):15217-15222. doi: 10.1073/pnas.1204705109

Fig. 3.

Fig. 3.

PEG-IPTG hydrogels. (A) IPTG (highlighted orange) was chemically attached to PEG chains via an ester bond using click chemistry. (B) Q-PCR of EGFP expression in the PEG-IPTG hydrogels. Gene expression of CHO cells stably transfected with LTRi_EGFP in PEG-IPTG hydrogels is compared to 250 pM IPTG induction in the media. All expression levels were normalized to GAPDH as the reference gene. (C) Bright field image of CHO cells stably transfected with LTRi_EGFP 3 d after encapsulation in PEG-IPTG (250 uM IPTG) gels. (C′) Fluorescent image of (C).