Fig. 5.
Proton-coupled antiport in CLCF homologues. (A) Aligment of the PIGG-pen sequence of functionally verified CLC Cl- channels or Cl-/H+ antiporters. Black or blue lettering indicate eukaryotic or bacterial homologues, respectively. Eukaryotic homologues are from mammals (CLC-1,-2,-7, -K1), elasmobranch (CLC-0), plants (atCLCa, cmCLC), yeast (GEF1), and amoeba (CLC-ehB). (B) F- gradient-driven H+ uptake traces shown are representative of the full set of homologues studied. H+ uptake was monitored by time-dependent pH increase of the suspension of liposomes reconstituted with the CLCF homologues indicated. Transport was started by Vln, and FCCP was added (arrows) after steady-state uptake had been reached.