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. 2012 Aug 27;109(38):E2543–E2550. doi: 10.1073/pnas.1205664109

Fig. 6.

Fig. 6.

Induction of p53 isoforms is mediated by the T4SS. (A) Protein lysates were prepared from control SNU-1 cells (−) or those co-cultured with H. pylori (Hp) (+) strain J166 at the indicated cell/bacteria ratios or with heat-inactivated bacteria and analyzed for expression of Δ133p53 protein by Western blotting. (Right) qPCR analysis of Δ133p53 transcripts in SNU-1 cells co-cultured with H. pylori is shown. Data are normalized to HPRT1 mRNA expression. The Δ133p53 mRNA level in uninfected cells is arbitrarily set at 1. (B) SNU-1 cells were co-cultured with WT H. pylori strain J166 or its isogenic cagA-, cagE-, or vacA-null mutants and analyzed for Δ133p53 mRNA by qPCR. SNU-1 cells were co-cultured with H. pylori as described in B for 12 h and analyzed for expression of p53 and Δ133p53 proteins (C), as well as c-Jun protein levels and its phosphorylation at position Ser63 (D), by Western blotting.