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Kb–SIIN and Kb–SIYR complexes exist in distinct subcellular compartments. (A) Four hours p.i. with VV-expressing Ub-liberated SIIN (MOI = 1), cells were stained intracellularly with 25D1.16 and antibodies against the indicated intracellular markers, followed by the appropriate secondary antibody staining. (B) Four hours p.i. with VVs expressing Ub-liberated SIYR and SIIN (each at MOI = 1), cells were stained intracellularly using 2C m67 and 25-D1.16.
