Fig. 7.

Tipe2^−/− mice are resistant to L. monocytogenes and S. aureus infection. (A) Survival rate of WT and Tipe2^−/− mice (n = 7) infected with 2 × 10⁵ cfus of L. monocytogenes (Lm) via the tail vein. The difference between the two groups is statistically significant (P < 0.01). (B) The numbers of bacteria in the spleen and liver of individual mice were determined on day 3 by colony-forming unit assay. (C) Levels of serum aspartate transaminase (AST) and alanine transaminase (ALT) on day 3. (D) One day after the infection, serum cytokines were determined by ELISA. Error bars represent the SDs of the means. (E) Survival rate of WT and Tipe2^−/− mice (n = 9) infected with S. aureus (6 × 10⁷ cfus/mouse) via the tail vein. (F) Bacterial titers were assayed at 24 h after i.v. inoculation of S. aureus (2 × 10⁷ cfus/mouse). Statistics were performed on pooled data from two independent experiments. *P < 0.05, **P < 0.01.