Fig. 1.
Influence of RpfFBc and RpfR on intracellular c-di-GMP level. (A) Detection of intracellular c-di-GMP level by HPLCy (HPLC) assay. Relative amount of c-di-GMP was calculated on the basis of their peak areas. For the convenience of comparison, c-di-GMP level of B. cenocepacia wild-type strain H111 was arbitrarily defined as 100% and used to normalize the c-di-GMP level ratios of the other strains. Data shown are means of two repeats and error bars indicate SDs. (B) Genomic organization of the region encoding RpfFBc and RpfR in B. cenocepacia H111 and domain structure analysis of RpfR.