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ITC analysis of the molecular interaction between BDSF and RpfR. (A) ITC titration of 20 μM RpfR with 200 μM BDSF in PBS buffer at 21 °C. (B) ITC titration of 20 μM RpfR with 200 μM of the saturated isomer of BDSF in PBS buffer at 21 °C.