Fig. 3.

ApAUF1 binds to the 3′ UTR of ApC/EBP through AREs. (A) Schematic structure of reporter RNAs. In ΔARE mutant, 448 bp containing putative six AREs in the 3′ UTR of ApC/EBP were deleted. (B) ApAUF1 interacts with intact 3′ UTR (wt), but not with ΔARE mutant RNA. In RNA-protein pull-down assay, biotin-labeled reporter RNA was incubated with lysate of HEK293T cells overexpressing 3x flag-ApAUF1. After pulling-down with avidin, RNA–protein complex was detected in Western blot by using an mFlag-m2 antibody.