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. 2012 Sep 4;109(38):15520–15525. doi: 10.1073/pnas.1116224109

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Fig. 5. — Blockade of 5-HT–induced ApC/EBP induction by ApAUF1 overexpression. (A) Sensory neurons expressing ApAUF1 and GFP, or GFP alone, were stimulated with five pulses of 5-HT, and ApC/EBP mRNA was detected by in situ hybridization. Digoxigenin-labeled antisense riboprobes against ApC/EBP were used to detect ApC/EBP transcripts. Sensory cells expressing only GFP showed a strong signal for ApC/EBP when they were stimulated by 5-HT (Left). However, ApAUF1 overexpressing neurons exhibited less staining after 5-HT stimulation (Right). (Scale bar: 50 μm.) (B) ApC/EBP expression was quantified by measuring pixel intensity from the cell bodies. Signal intensities were significantly lower in ApAUF1 overexpressing neurons compared with GFP expressing control neurons (arbitrary unit of signal intensity, ApAUF1 + 5× 5-HT, 0.835 ± 1.065, n = 9; GFP + 5× 5-HT, 14.310 ± 2.076, n = 19; t test, ***P < 0.001).