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Lysates of YT cells preincubated for 3 h with control medium (lanes a and c) or with 10 μM MG132 (lane b) were immunoprecipitated with an anti-IL2Rβ antibody (lanes a and b) or with a mouse monoclonal anti-ubiquitin antibody (lane c) and analyzed by immunoblotting with anti-IL2Rβ antibody. The upper arrow indicates a ubiquitinated form of the IL2Rβ chain migrating at 78 kDa.
