Table 2.
Gene | Product name | Primer | Direction | Sequence (5’–3’) | Reference |
---|---|---|---|---|---|
ACT | Actin | ACT-512F | Foward | ATG TGC AAG GCC GGT TTC GC | Carbone & Kohn 1999 |
ACT-783R | Reverse | TAC GAG TCC TTC TGG CCC AT | Carbone & Kohn 1999 | ||
CAL | Calmodulin | CL1 | Foward | GAR TWC AAG GAG GCC TTC TC | O’Donnell et al. 2000 |
CL2A | Reverse | TTT TTG CAT CAT GAG TTG GAC | O’Donnell et al. 2000 | ||
CL1C | Foward | GAA TTC AAG GAG GCC TTC TC | This study | ||
CL2C | Reverse | CTT CTG CAT CAT GAG CTG GAC | This study | ||
CHS-1 | Chitin synthase | CHS-79F | Foward | TGG GGC AAG GAT GCT TGG AAG AAG | Carbone & Kohn 1999 |
CHS-345R | Reverse | TGG AAG AAC CAT CTG TGA GAG TTG | Carbone & Kohn 1999 | ||
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | GDF | Foward | GCC GTC AAC GAC CCC TTC ATT GA | Templeton et al. 1992 |
GDR | Reverse | GGG TGG AGT CGT ACT TGA GCA TGT | Templeton et al. 1992 | ||
GS | Glutamine synthetase | GSF1 | Foward | ATG GCC GAG TAC ATC TGG | Stephenson et al. 1997 |
GSF3 | Foward | GCC GGT GGA GGA ACC GTC G | This study | ||
GSR1 | Reverse | GAA CCG TCG AAG TTC CAG | Stephenson et al. 1997 | ||
GSR2 | Reverse | GAA CCG TCG AAG TTC CAC | This study | ||
ITS | Internal transcribed spacer | ITS-1F | Foward | CTT GGT CAT TTA GAG GAA GTA A | Gardes & Bruns 1993 |
ITS-4 | Reverse | TCC TCC GCT TAT TGA TAT GC | White et al. 1990 | ||
SOD2 | Manganese-superoxide dismutase | SODglo2-F | Foward | CAG ATC ATG GAG CTG CAC CA | Moriwaki & Tsukiboshi 2009 |
SODglo2-R | Reverse | TAG TAC GCG TGC TCG GAC AT | Moriwaki & Tsukiboshi 2009 | ||
TUB2 | β-Tubulin 2 | T1 | Foward | AAC ATG CGT GAG ATT GTA AGT | O’Donnell & Cigelnik 1997 |
T2 | Reverse | TAG TGA CCC TTG GCC CAGT TG | O’Donnell & Cigelnik 1997 | ||
Bt2b | Reverse | ACC CTC AGT GTA GTG ACC CTT GGC | Glass & Donaldson 1995 |