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. Author manuscript; available in PMC: 2012 Sep 26.
Published in final edited form as: J Immunol. 2010 Jan 4;184(3):1218–1226. doi: 10.4049/jimmunol.0902620

Figure 2.

Figure 2

The effects of Sphingomonas spp. in RF intestinal enteric microbiota on iNKT cells. The colonization of Sphingomonas spp. in CF versus RF mice was assayed by PCR for Sphingomonas 16r DNA, and the effects of intestinal inoculation of Sphingomonas on iNKT cells were evaluated in GF mice. A, Sphingomonas-selective PCR of samples from the small and large intestinal mucosal compartments of CF and RF mice. Gel images show the detection of Sphingomonas spp. from four CF versus four RF mice. Arrow indicates amplicons of the anticipated size (303 bp). kb = 1 Kb DNA Ladder (Invitrogen, Carlsbad, CA). Int, intestine. B, Effect of intragastic challenge with S. yanoikuyae on iNKT cells in GF mice. Relative iNKT cell numbers in indicated organs are shown at 24 h after intragastic treatment with PBS (control, squares, n = 4) or S. yanoikuyae (S.yanoi, circles, n = 5). p(liver) = 0.01, p (spleen) = 0.022. C, Expression of CD69 or CD25 on liver iNKT cells from control (tined) or S. yanoikuyae-challenged mice (bold).