Skip to main content
. 2012 Aug 16;23(10):1652–1662. doi: 10.1681/ASN.2012030285

Figure 3.

Figure 3.

Activation of Akt results in accumulation of fibronectin in renal primary proximal tubular cells. (A) Cell lysates of primary proximal tubular cells isolated from TSC2+/− and wild-type cells were subjected to Western blot analysis. Increases in phospho-Akt are associated with increased fibronectin expression in TSC2+/− rats compared with wild-type rats. (B) Histograms represent mean ± SEM of three experiments. **P<0.01, significant difference from wild-type cells. (C and D) Inhibition of Akt blocks fibronectin expression. TSC2+/− and wild-type cells were treated with different concentrations of Akt inhibitor IV (0–10 μM) for 24 hours show a significant decrease in fibronectin protein expression. The inhibition of Akt was associated with a decrease in fibronectin expression and was dose dependent. Note that Akt phosphorylation is more prominent in cells partially deficient in tuberin (TSC2+/−) compared with wild-type cells.