Figure 1.

CFTR colocalizes with BiP in yeast and accumulates at one or two sites in the proteasome mutant strain at 40°C. CFTR-expressing ssa1-45 (A) and pre1-1pre2-2(B) cells and the respective isogenic wild-type cells (SSA1 and PRE) were grown at 26°C, shifted to 40°C for 1 h, and then subjected to indirect immunofluorescence microscopy with anti-HA (to detect HA-CFTR) and anti-BiP antibodies.