Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jun 21;40(17):8698–8710. doi: 10.1093/nar/gks582

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — VcHfq preferentially binds Qrr1 with its proximal face. Representative EMSAs for a distal face mutant (Y25D), a proximal face mutant (K56A) and a distal and proximal face double mutant (Y25D K56A) of VcHfq binding to Qrr1 sRNA. Qrr1 was labelled with ³²P and was present at 5 nM in each lane and the concentration of hexameric VcHfq protein is specified above the lane. The mobilities of free Qrr1 RNA, the 1:1 VcHfq:Qrr1 complex and higher order complexes are indicated on the right of the gels. The dissociation constant (K_d) for the 1:1 VcHfq:Qrr1 complex, calculated from three repeats, is reported above each gel.