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. 2012 Jun 21;40(17):8472–8483. doi: 10.1093/nar/gks583

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) P_tet_A-mRFP1-96bs-BAC plasmid map: nucleotide sequence of the tet regulatory region on the BAC plasmid, beginning with P_tetA (-35 and −10 consensus sequences), TSS (+1), palindromic patterns corresponding to two TetR binding sites (blue), mRFP1 start site at +104, and 96bs start site at +778. (B) P_N25-tetR sequence integrated in the E. coli genome: P_N25 controlled TetR protein coding gene and the lambda attP site. Terminators t₀ and T1 prevent transcription from the integrated promoters into the neighbouring regions of the E. coli genome.