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. 2012 Jun 28;40(17):8773–8781. doi: 10.1093/nar/gks597

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Modularity of T7 promoters. (A) A library of pT7 promoters of differing strengths was created by mutating the strength-determining region (−2bp to +3bp) and cloned into plasmid N155 (Supplementary Data). Synonymous mutations were also made to the pT3 promoter. Sequences are shown for the two libraries. (B) The promoter libraries were used to control fluorescent reporter proteins, and each library was co-transformed with both T7* and T7*(T3). Activity of co-transformants under 1 mM IPTG induction was characterized by flow cytometry to study cognate interactions (T7*-P_T7, top left and T7*(T3)-P_T3, bottom right) and non-cognate interactions (T7*-P_T3, bottom left and T7*(T3)-P_T7, top right). Error bars represent the standard deviation of three experiments on different days.