Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jun 22;40(17):8484–8498. doi: 10.1093/nar/gks598

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — CPEB3 binds to the C-terminus of IPO5 through its RRM1. (A) The domain organization of CPEB3 and various truncated mutants. (B) Co-IP assay. HeLa cells were co-transfected with EGFP-IPO5 plasmid along with various myc-tagged CPEB3 constructs. The cell lysates were immunoprecipitated with GFP or myc antibody, and immunoblotted with myc and GFP antibodies. IP, immunoprecipitation; IB, immunoblotting. (C) The domain organization of IPO5 and various deleted mutants. NPC, nuclear pore complex. (D) HeLa cells expressing myc-CPEB3 along with various EGFP-tagged wt and mutant IPO5 proteins were pulled down by myc or GFP antibody. The precipitated substances were immunodetected with myc and GFP antibodies.