Figure 5.

Cells lacking the RecQ-helicase Sgs1 increase the sensitivity to agents that cause DNA damage or replicative stress in the absence of Mms4 phosphorylation. Serial dilutions (10-fold) of normalized log-phase cultures of the different strains were spotted onto YPD plates containing the indicated amounts of MMS, 4NQO, HU or cisplatin and incubated at 30°C for 48–72 h. The HA-MMS4 and sgs1ΔHA-MMS4 strains, which behave like wild-type and sgs1Δ, respectively, were used as controls to show that the phenotypes of sgs1Δ-HA-mms4-np cells are not due to the tag on Mms4.