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. 2012 Jun 22;40(17):8336–8347. doi: 10.1093/nar/gks604

Figure 1.

Figure 1.

Similarly enhanced radiosensitivity in AT and Artemis fibroblasts due to an enhanced number of residual DSBs. (A) Radiosensitivity of exponentially growing WT, AT and Artemis cells (FO2-385) was measured by colony formation after various X-ray doses. Error bars represent the SEM of three independent experiments. (B) Differential staining for G2-phase cells (Cenp-F-positive) 24 h after irradiation with 1 Gy in addition to detection of γH2AX foci. Bars, 10 µm. Quantification of residual γH2AX foci in (C) Cenp-F-negative G1-phase cells and (D) Cenp-F-positive G2-phase cells 24 h after IR (1 Gy). S-phase cells showing a strong pan-nuclear γH2AX signal were excluded from the analysis. The AT cell line AT1BR proved to be hyperploid (1.5-fold enhanced compared with WT and Artemis cells (data not shown)). Their foci number/nucleus was normalized to a diploid DNA content.