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. 2012 Jun 22;40(17):8336–8347. doi: 10.1093/nar/gks604

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The number of Rad51 foci in G2 is elevated in AT but not in Artemis cells. (A) Differential staining for G2-phase cells (Cenp-F-positive) in addition to detection of Rad51. Rad51 foci were visible only in Cenp-F-positive G2-phase cells (left panel). Magnification revealed distinct Rad51 foci (yellow as merged from green and red). The right panel depicts the red channel only as recorded in black/white. (B) Cells were co-stained for γH2AX and Rad51, which showed that in AT cells all Rad51 foci co-localized with γH2AX but not in both Artemis cell lines. Shown are cells in G2. G1 cells did not stain for Rad51 and S-phase cells with hyperintense γH2AX signals were not considered. Bars, 10 µm. (C) Quantification of Rad51 and γH2AX foci 24 h after IR revealed identical numbers for both damage markers in WT and AT but not in both Artemis cell lines.