Skip to main content
. 2012 Jun 22;40(17):8336–8347. doi: 10.1093/nar/gks604

Figure 4.

Figure 4.

HR during the S-phase is dependent on ATM but not Artemis. (A) WT, AT and FO2-385 cells were EdU pulse-labeled, irradiated with 1 Gy and further incubated with aphidicoline (+Aphi) (5 µM) to block the transition of S-phase cells into G2. Kinetics of Rad51 foci numbers were monitored in Cenp-F-positive and EdU-negative G2 cells for the time interval indicated. (B) WT, AT and the Artemis cell lines FO2-385, CJ179 were EdU pulse-labeled, irradiated (1 Gy) and kinetics of Rad51 foci were evaluated in EdU-positive cells. (C) Upon ATM inhibition (10 µM KU55933), all cell lines showed similar kinetics of Rad51 foci. (D) WT, AT and both Artemis cell lines were seeded for colony formation, irradiated with 1, 2 or 4 Gy as indicated and incubated for 24 h in the presence or absence of 10 µM KU55933. Data without ATM inhibitor (black open symbols, dashed lines) were taken from Figure 1. WT and both Artemis cell lines but not AT cells were sensitized by the ATM inhibitor KU55933 (black closed symbols). Co-treatment with 5 µM aphidicolin (gray closed symbols) partially relieved this sensitization.