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. 2012 Jun 25;40(17):8646–8661. doi: 10.1093/nar/gks609

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — All the B-factors are necessary to recruit Nog2 to pre-ribosomes; only a subset of B-factors are required to recruit Nsa2 into pre-ribosomes. (A) The changes in the levels of pre-ribosome-associated Nsa2 and Nog2 in the absence of each of the B-factors. Pre-ribosomes were purified from cells grown in YEPGal or shifted to YEPGlu to deplete each B-factor. Proteins from pre-rRNPs were resolved by SDS–PAGE and assayed by western blotting. In each pair of samples shown in this figure and in Figures 4–7, protein from the undepleted strain is on the left and protein from the depleted strain is on the right. In this blot and all subsequent blots, where two bands are detected, the bona fide protein is indicated with an asterisk. Nop7-TAP serves as the loading control. (B) Nog2 is not required to recruit any of the B-factors. Proteins in pre-ribosomes purified from GAL-NOG2 yeast grown in YEPGal, or shifted to YEPGlu to deplete Nog2 were assayed by western blotting. Nop7-TAP serves as a loading control. Each pair of samples is shown as described in Figure 3A. (C) All of the B-factors are required to recruit Nog2 to pre-ribosomes. A subset of B-factors is required to recruit Nsa2 and Nog2.