Skip to main content
. 2012 Jun 25;40(17):8348–8360. doi: 10.1093/nar/gks612

Figure 5.

Figure 5.

Impaired DNA replication-coupled homologous recombination in p21−/− cells. HCT116 p21+/+ and p21−/− cells were incubated in the presence of bromodeoxyuridine for two cell cycles and in the absence or presence of mitomycin C (A), camptothecin (B) or etoposide (C) for the second cell cycle period. Metaphase spreads were prepared, and the numbers of SCEs were scored. Error bars represent the standard errors of the means. At least 30 metaphases were scored per treatment per experiment. (D) HR reporter assay for the measurement of replication-dependent HR repair of a site-specific DNA ICL (9). (E) p21+/+ and p21−/− cells were transiently transfected with the TR-OriP-GFP vector alone (OriP), TR-OriP-GFP without an ICL (TFO) and psoralen-UVA crosslinked TR-OriP-GFP (pso-TFO), in the presence of EBNA1. Error bars represent standard errors of the means from three independent experiments.*P < 0.05; **P < 0.01; ***P < 0.001.