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. 2012 Jun 25;40(17):8593–8606. doi: 10.1093/nar/gks613

Figure 3.

Figure 3.

Mapping, phenotype and complementation of rhon1. (A) Molecular markers (H-M) used for fine mapping of rhon1 on chromosome 1 and the corresponding numbers of recombinant events are shown. Sequencing revealed that the T-DNA was inserted into the At1g06190 gene (RHON1). (B) Exons (grey boxes) and introns (black lines) show the structure of the genomic RHON1 gene. The T-DNA insertion at position +481 is marked. Arrows indicate the positions of primers used for PCR analysis. (C) RHON1 contains a putative chloroplast transit peptide (TP), two highly conserved regions (red) and the C-terminal motif Rho-N (yellow). For complementation of the mutant, a construct of the full-length protein fused to an HA-Strep TAP-tag was used. (D) WT and complemented rhon1TAP plants were grown on soil for 4 weeks in 12/12 hours light/dark conditions. Scale bars indicate the size of 1 cm. (E) PCR analysis demonstrates orientation of the T-DNA insertion in exon 2 of rhon1 and homozygosity of independent complemented lines. (F) Chlorophyll fluorescence was imaged from protoplasts isolated from WT, rne-1 and rhon1. Scale bars indicate the size of 10 µm.