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. 2012 Jun 25;40(17):8593–8606. doi: 10.1093/nar/gks613

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — RNE levels in rhon1 and the phenotype of rne/rne rhon1/rhon1 double knockouts. (A) Comparison of RNE levels in wild type, rne and rhon1 mutants. Levels of the protein PAC were used as a loading control. (B) Double knockouts were generated by crossing heterozygous rne-1 and rhon1 mutants and selfing of the selected double heterozygous F1. The average Fv/Fm rate and standard deviation were determined from 10 independent plants. Representative mutant plants are shown above each column, whereas wild-type and double heterozygous plants are only shown in parts to maintain the same scale.